General description

A cell-permeable dinitrobromobenzene compound that acts as a selective inhibitor of yeast Endoplasmic Reticulum Oxidase 1 (ERO1), but has somewhat weaker activity against mouse ERO1α (IC₅₀ = 400 µM). Predicted to form thiol adducts and inhibits ERO1-dependent oxidation of thioredoxin-1 (Trx1) activity in vitro. Shown to cluster with genes involved in protein folding, glycosylation and cell wall biosynthesis, and delay carboxypeptidase Y (CPY) processing to the vacuolar form in wt-yeast cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Costanzo, M., et al. 2010. Science327, 425.Blais, J.D., et al. 2010. J. Biol. Chem.285, 20993.

Packaging

25 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze(-20°C). Stock solutions are stable for up to 1 month at -20°C.

Warning

Toxicity: Standard Handling (A)